microbiology lectures pdf

Extreme Environments er'of'viable'cells'versus'incubation'time. Thissuperior illumination scheme is still widely used in modern microscopes and forms the basis forphase contrast, differential interference contrast, epifluorescence, and confocal microscopy, Lecture 2: The Microscope 1. - Spirilla-­‐ rigid helices. Energy: to fuel metabolic reactions - Phototroph: Use light for energy Thanks. Chromosome replication disease but absent in healthy organisms 2. Psychrophiles: Love cold temperatures. 10 1'cells ' 9 ' 'etc '' phagocytosis'inhibitors,'and'' 2. 4. Initially taxonomy was based on phenotypic/physical structure. Lecture 2: Chemical Principles - Bond types, reactions, organic compounds Lecture 2 handout. ,', Microbiology Disinfection The killing, inhibition or removal of pathogenic organisms. Modify, remix, and reuse (just remember to cite OCW as the source. Protein'synthesis' proteins'can'form'a'channel'in'the'outer' LEC # TOPICS LECTURERS SLIDES NOTES; 1: Early Earth/Microbial … Bacterial Essential ElementsThe following elements are required in large amounts in bacterial cells as they are used to build upall the necessary macromolecules for survival. The same disease must result when the isolated microorganism is inoculated into a healthy host. The cell is able to unwind DNA as required for gene expression. (gram negative must secrete across 2 membranes). 10 3'cells ' 7 ' '99.9999%' &'Gram'negative'bacteria,'make'use'of'ABC'protein' Elongation Comments. lackofnutrients,reactivatewhennutrientsaredetected.Anthraxisanexampleofdried,milledsporesthatcanbeingestedandbecomeactiveasthehumanbod ypresentsidealnutrientconditions. - Nitrogen: Used to build amino acids, nucleotides and enzyme factors. Microbiology at Wilkes University . Growth Factors Lecture 1: Classification and history Microbial Death Cell'structure', Lipid'structure' Endosymbiosis Hypothesis “Static” To stop or inhibit. Principle Definition Most prokaryotes undergo binary fission where DNA is replicated and undergoes cytokinesis. Archaea are responsible for the production of methane underground. Euryarchaeota: Very diverse and large group Microbiology & Microorganism Learning Objective: Knows the basic knowledge of microbes. Spontaneous generation: The idea that living organisms could develop from non-­‐living matter. Compare the theories of spontaneous generation and biogenesis. Lectures : Lecture Notes . 2. - Thermophilic sulfate reducers, Crenarchaeota: originally isolated from hot springs Use OCW to guide your own life-long learning, or to teach others. Your use of the MIT OpenCourseWare site and materials is subject to our Creative Commons License and other terms of use. Preparation of Microbial Specimens 'they'have'shared'properties)'', Lecture 4: AchaeaLecture 4: Microbial Evolution and Archaea Freely browse and use OCW materials at your own pace. - Can easily and readily move between bacteria. - Animalia Mitochondrial and chloroplast chromosomes bear similarity to current bacteria and cyanobacteria Find materials for this course in the pages linked along the left. (Rather a historical term). - Brightfield: dark object, bright background. Massachusetts Institute of Technology. Stages of Growth curve Lab Reviews (htm) Unit 1: Unit 2: Unit 3: Unit 4 : Unit 5: Study Guides: Laboratory Reviews: Laboratory Images; Bacterial Morphology; Bacterial Stains; Fungal Spores - Asexual; Fungal Spores - Sexual; Differential (Biochemical) Media. PV. Control Methods - Protein sequence (Gram staining irrelevant) » Eukarya Light MicroscopyLight microscopy uses different properties of light passing though thespecimen to visualise the structure and infers function. Once the population has increased in sizequo rum sensing occurs where the bacterial cells sense (using AHL) they have a big enough population toinfect a bigger organism. Archea - Prokaryotes The 3 Domain SystemThis phylogenetic tree is based on single subunit rRNA(SSU rRNA). There were several challenges to this theory - Agostini Bassi: diseases were due to microbial infections. (Phosphates,sulfur,carbon,Iron) - DNA/RNA sequences The modification maintains theconcentration gradient allowing molecules (sugars) tocontinue to move into the cell. - Carry genes that give a selective trait. Production of methane (derive energy). Microbiology Lecture Notes and Study Guides Microsoft Word 7 - .doc files. 2. Nonew nutrients are added. Kohler Illumination Sec B is a chaperone that delays the protein folding keeping the peptide chain linear. Send to friends and colleagues. 3. Peizophiles: Tolerate high pressure. The biofilmforms a community sharing nutrients and waste products. On a plate the most rapid growth occurs at the edge (youngest cells) where more nutrients and oxygen are available. The energy (power)system created could maintain larger, more complex genomes. 14'proteins'integral'membrane'proteins.' There are no known archaeal pathogens. Electrons: to play a role in energy production 10 <4'cells '14' 4. Strains: Descendent from a single pure microbial culture - Biovars are different biochemically and physiologically - Morphovars are different morphologically - Serovars are different antigenically (not recognized by the same antibodies). - The terminus is the site at which replication is terminated. Granulesoforganicorinorganicmaterialwithinthecytoplasmicmatrix.Theystoreawaynutrientsthatarelimitedintheenvironmentingranulesforfuturesurvival . Principles of Control Helpful? - Distinctive RNA sequence - Lack peptidoglycan in cell wall Unusual'shapes'too', Methanobacterium 11 These agents do not kill, but they do prevent growth. Rods'and'cocci'common' Population size: A larger population or microorganism requires a longer time of exposure to be killed then a smaller one. - Manganese, zinc, cobalt, nickel and copper are required in trace amounts for some enzymatic function or protein stabilization. ' e.g. Type Strain: The first strain of a species to be studied, usually it is the most fully characterized but doesnot mean it is the most abundant. - Lithotroph: use reduced mineral (inorganic) source for electrons - Organotroph: extract electrons from organic molecules. 4. Hydrogen Hypothesis Please sign in or register to post comments. This can stop growth long enough for the immune system to destroy cells. 141 16. Bacteria Taxonomy is the science of classifying microorganisms. - Can survive extreme environment conditions - No pathogenic species known. RNA'synthesis' About. The growth curve oforganisms dividing by binary fission is the logarithmicplot of the number of viable cells vs incubation time. thermoautotrophicum1 Haloquadratum1walsbyi1 Thermoproteus1tenax1 Haloarcula1japonica, Tetra

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